› 1ys7

X-ray diffraction
1.58Å resolution

Crystal structure of the response regulator protein prrA complexed with Mg2+

Released:
DOI: 10.2210/pdb1ys7/pdb
PDB entry 1ys7 coloured by chain, front view.
PDB entry 1ys7 coloured by chain, side view.
PDB entry 1ys7 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
The structural basis of signal transduction for the response regulator PrrA from Mycobacterium tuberculosis.
Nowak E, Panjikar S, Konarev P, Svergun DI, Tucker PA
J Biol Chem 281 9659-66 (2006)
PMID: 16434396

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161500 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulatory protein PrrA Chains: A, B
Molecule details ›
Chains: A, B
Length: 233 amino acids
Theoretical weight: 25.04 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WGM1 (Residues: 1-233; Coverage: 100%)
Gene names: MTCY31.31c, Rv0903c, prrA
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand MG 2 x MG
Ligand ACT 2 x ACT
Ligand TRS 1 x TRS
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P1
Unit cell:
a: 37.35Å b: 46.486Å c: 65.554Å
α: 100.33° β: 102.01° γ: 92.9°
R-values:
R R work R free
0.189 0.187 0.23
Expression system: Escherichia coli

Quick links

Citations

9 review citations

X-ray solution scattering (SAXS) combined with crystallography and computation: defining accurate macromolecular structures, conformations and assemblies in solution.
Putnam et al. (2007)
8 more

3 mentions without citation

Structure of the DNA-binding domain of the response regulator PhoP from Mycobacterium tuberculosis.
Wang et al. (2007)
2 more