› 1yub

Solution NMR

SOLUTION STRUCTURE OF AN RRNA METHYLTRANSFERASE (ERMAM) THAT CONFERS MACROLIDE-LINCOSAMIDE-STREPTOGRAMIN ANTIBIOTIC RESISTANCE, NMR, MINIMIZED AVERAGE STRUCTURE

Released:
DOI: 10.2210/pdb1yub/pdb
PDB entry 1yub coloured by chain, front view.
PDB entry 1yub coloured by chain, side view.
PDB entry 1yub coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
Solution structure of an rRNA methyltransferase (ErmAM) that confers macrolide-lincosamide-streptogramin antibiotic resistance.
Yu L, Petros AM, Schnuchel A, Zhong P, Severin JM, Walter K, Holzman TF, Fesik SW
Nat Struct Biol 4 483-9 (1997)
PMID: 9187657

Function and Biology Details

Reaction catalysed: 
2 S-adenosyl-L-methionine + adenine(2085) in 23S rRNA = 2 S-adenosyl-L-homocysteine + N(6)-dimethyladenine(2085) in 23S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149128 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
rRNA adenine N-6-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 28.86 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21236 (Residues: 1-245; Coverage: 100%)
Gene name: erm
Sequence domains: Ribosomal RNA adenine dimethylase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: DG/SA
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

The use of 2H, 13C, 15N multidimensional NMR to study the structure and dynamics of proteins.
Gardner et al. (1998)
7 more

11 mentions without citation

Many paths to methyltransfer: a chronicle of convergence.
Schubert et al. (2003)
10 more