› 1yv3

X-ray diffraction
2Å resolution

The structural basis of blebbistatin inhibition and specificity for myosin II

Released:
DOI: 10.2210/pdb1yv3/pdb
PDB entry 1yv3 coloured by chain, front view.
PDB entry 1yv3 coloured by chain, side view.
PDB entry 1yv3 coloured by chain, top view.
Source organism: Dictyostelium discoideum
Primary publication:
The structural basis of blebbistatin inhibition and specificity for myosin II.
Allingham JS, Smith R, Rayment I
Nat Struct Mol Biol 12 378-9 (2005)
PMID: 15750603

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Myosin-2 heavy chain Chain: A
Molecule details ›
Chain: A
Length: 762 amino acids
Theoretical weight: 86.75 KDa
Source organism: Dictyostelium discoideum
UniProt:
  • Canonical: P08799 (Residues: 2-759; Coverage: 36%)
Gene names: DDB_G0286355, mhcA
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand MG 2 x MG
Ligand VO4 1 x VO4
Ligand ADP 1 x ADP
Ligand BIT 1 x BIT
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: C2221
Unit cell:
a: 88.106Å b: 145.77Å c: 152.912Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.173 0.215

Quick links

Citations

23 review citations

Nonmuscle myosin-2: mix and match.
Heissler et al. (2013)
22 more

25 mentions without citation

Variability in docking success rates due to dataset preparation.
Corbeil et al. (2012)
24 more