› 1yvs

X-ray diffraction
2.2Å resolution

Trimeric domain swapped barnase

Released:
DOI: 10.2210/pdb1yvs/pdb
PDB entry 1yvs coloured by chain, front view.
PDB entry 1yvs coloured by chain, side view.
PDB entry 1yvs coloured by chain, top view.
Source organism: Bacillus amyloliquefaciens
Primary publication:
Trimeric domain-swapped barnase.
Zegers I, Deswarte J, Wyns L
Proc Natl Acad Sci U S A 96 818-22 (1999)
PMID: 9927651

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo trimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-132805 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease Chain: A
Molecule details ›
Chain: A
Length: 110 amino acids
Theoretical weight: 12.4 KDa
Source organism: Bacillus amyloliquefaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00648 (Residues: 48-157; Coverage: 89%)
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: R32
Unit cell:
a: 70.08Å b: 70.08Å c: 162.55Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.187 0.187 0.244
Expression system: Escherichia coli

Quick links

Citations

7 review citations

3D domain swapping: as domains continue to swap.
Liu et al. (2002)
6 more

6 mentions without citation

Biological Activities of Secretory RNases: Focus on Their Oligomerization to Design Antitumor Drugs.
Gotte et al. (2019)
5 more