1ywa

X-ray diffraction
0.89Å resolution

0.9 A Structure of NP4 from Rhodnius Prolixus complexed with CO at pH 5.6

Released:

Function and Biology Details

Reaction catalysed:
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H(2)O
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-4 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.29 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q94734 (Residues: 22-205; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2
Unit cell:
a: 70.079Å b: 42.587Å c: 52.998Å
α: 90° β: 94.36° γ: 90°
R-values:
R R work R free
0.121 0.12 0.15
Expression system: Escherichia coli BL21(DE3)