› 1ywd

X-ray diffraction
1.08Å resolution

1.08 A Structure of Ferrous NP4 (aquo complex)

Released:
DOI: 10.2210/pdb1ywd/pdb
PDB entry 1ywd coloured by chain, front view.
PDB entry 1ywd coloured by chain, side view.
PDB entry 1ywd coloured by chain, top view.
Source organism: Rhodnius prolixus
Primary publication:
Ultrahigh resolution structures of nitrophorin 4: heme distortion in ferrous CO and NO complexes.
Maes EM, Roberts SA, Weichsel A, Montfort WR
Biochemistry 44 12690-9 (2005)
PMID: 16171383

Function and Biology Details

Reaction catalysed: 
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-4 Chain: A
Molecule details ›
Chain: A
Length: 184 amino acids
Theoretical weight: 20.29 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q94734 (Residues: 22-205; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: C2
Unit cell:
a: 70.063Å b: 42.905Å c: 52.272Å
α: 90° β: 94.37° γ: 90°
R-values:
R R work R free
0.141 0.14 0.17
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Small ligand-globin interactions: reviewing lessons derived from computer simulation.
Capece et al. (2013)
2 more

3 mentions without citation

The structure and NO binding properties of the nitrophorin-like heme-binding protein from Arabidopsis thaliana gene locus At1g79260.1.
Bianchetti et al. (2010)
2 more