› 1ywk

X-ray diffraction
2.95Å resolution

Crystal structure of 4-deoxy-1-threo-5-hexosulose-uronate ketol-isomerase from Enterococcus faecalis

Released:
DOI: 10.2210/pdb1ywk/pdb
PDB entry 1ywk coloured by chain, front view.
PDB entry 1ywk coloured by chain, side view.
PDB entry 1ywk coloured by chain, top view.
Source organism: Enterococcus faecalis
Entry authors: Fedorov AA, Fedorov EV, Almo SC, Burley SK, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
5-dehydro-4-deoxy-D-glucuronate = 3-deoxy-D-glycero-2,5-hexodiulosonate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-182875 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase 1 Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 289 amino acids
Theoretical weight: 33.37 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q838L9 (Residues: 1-276; Coverage: 100%)
Gene names: EF_0425, kduI-1, kduI1
Sequence domains: KduI/IolB family
Structure domains: Jelly Rolls

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P212121
Unit cell:
a: 81.01Å b: 107.685Å c: 191.021Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.241 0.265
Expression system: Escherichia coli

Quick links