› 1yyo

X-ray diffraction
2.9Å resolution

Crystal structure of RNase III mutant E110K from Aquifex aeolicus complexed with double-stranded RNA at 2.9-Angstrom Resolution

Released:
DOI: 10.2210/pdb1yyo/pdb
PDB entry 1yyo coloured by chain, front view.
PDB entry 1yyo coloured by chain, side view.
PDB entry 1yyo coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Intermediate states of ribonuclease III in complex with double-stranded RNA.
Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X
Structure 13 1435-42 (2005)
PMID: 16216575

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130432 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Ribonuclease 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 26.15 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67082 (Residues: 1-221; Coverage: 100%)
Gene names: aq_946, rnc
Sequence domains:
Structure domains:
5'-R(*CP*GP*CP*GP*AP*AP*UP*UP*CP*GP*CP*G)-3' Chains: C, D, E, F
Molecule details ›
Chains: C, D, E, F
Length: 12 nucleotides
Theoretical weight: 3.83 KDa
Source organism: Aquifex aeolicus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 84.486Å b: 61.625Å c: 131.114Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.223 0.292
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

5 review citations

Ribonuclease revisited: structural insights into ribonuclease III family enzymes.
MacRae et al. (2007)
4 more

9 mentions without citation

RNA recognition by double-stranded RNA binding domains: a matter of shape and sequence.
Masliah et al. (2013)
8 more