› 1yzw

X-ray diffraction
2.1Å resolution

The 2.1A Crystal Structure of the Far-red Fluorescent Protein HcRed: Inherent Conformational Flexibility of the Chromophore

Released:
DOI: 10.2210/pdb1yzw/pdb
PDB entry 1yzw coloured by chain, front view.
PDB entry 1yzw coloured by chain, side view.
PDB entry 1yzw coloured by chain, top view.
Source organism: Heteractis crispa
Primary publication:
The 2.1A crystal structure of the far-red fluorescent protein HcRed: inherent conformational flexibility of the chromophore.
Wilmann PG, Petersen J, Pettikiriarachchi A, Buckle AM, Smith SC, Olsen S, Perugini MA, Devenish RJ, Prescott M, Rossjohn J
J Mol Biol 349 223-37 (2005)
PMID: 15876379

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188277 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GFP-like non-fluorescent chromoprotein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 225 amino acids
Theoretical weight: 25.65 KDa
Source organism: Heteractis crispa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q95W85 (Residues: 1-227; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand PEG 2 x PEG
1 modified residue:
Ligand CRU 4 x CRU

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 76.221Å b: 120.733Å c: 118.469Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.266 0.223 0.266
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
4 more

4 mentions without citation

Chromophore transformations in red fluorescent proteins.
Subach et al. (2012)
3 more