› 1z0s

X-ray diffraction
1.7Å resolution

Crystal structure of an NAD kinase from Archaeoglobus fulgidus in complex with ATP

Released:
DOI: 10.2210/pdb1z0s/pdb
PDB entry 1z0s coloured by chain, front view.
PDB entry 1z0s coloured by chain, side view.
PDB entry 1z0s coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Crystal structures of an NAD kinase from Archaeoglobus fulgidus in complex with ATP, NAD, or NADP.
Liu J, Lou Y, Yokota H, Adams PD, Kim R, Kim SH
J Mol Biol 354 289-303 (2005)
PMID: 16242716

Function and Biology Details

Reaction catalysed: 
ATP + NAD(+) = ADP + NADP(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128238 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD kinase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 278 amino acids
Theoretical weight: 31.04 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O30297 (Residues: 1-249; Coverage: 100%)
Gene names: AF_2373, nadK
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 4 x MG
Ligand ATP 4 x ATP
Ligand POP 4 x POP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P1
Unit cell:
a: 55.252Å b: 69.731Å c: 76.287Å
α: 106.35° β: 111.32° γ: 101.3°
R-values:
R R work R free
0.201 0.2 0.231
Expression system: Escherichia coli

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Citations

6 review citations

The power to reduce: pyridine nucleotides--small molecules with a multitude of functions.
Pollak et al. (2007)
5 more

3 mentions without citation

Analysis of the Staphylococcus aureus DgkB structure reveals a common catalytic mechanism for the soluble diacylglycerol kinases.
Miller et al. (2008)
2 more