› 1z0u

X-ray diffraction
2Å resolution

Crystal structure of a NAD kinase from Archaeoglobus fulgidus bound by NADP

Released:
DOI: 10.2210/pdb1z0u/pdb
PDB entry 1z0u coloured by chain, front view.
PDB entry 1z0u coloured by chain, side view.
PDB entry 1z0u coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Crystal structures of an NAD kinase from Archaeoglobus fulgidus in complex with ATP, NAD, or NADP.
Liu J, Lou Y, Yokota H, Adams PD, Kim R, Kim SH
J Mol Biol 354 289-303 (2005)
PMID: 16242716

Function and Biology Details

Reaction catalysed: 
ATP + NAD(+) = ADP + NADP(+)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128238 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 278 amino acids
Theoretical weight: 31.04 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O30297 (Residues: 1-249; Coverage: 100%)
Gene names: AF_2373, nadK
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21212
Unit cell:
a: 62.961Å b: 76.384Å c: 120Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.264
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The power to reduce: pyridine nucleotides--small molecules with a multitude of functions.
Pollak et al. (2007)
5 more

1 mention without citation

Enhanced degradation of dihydrofolate reductase through inhibition of NAD kinase by nicotinamide analogs.
Hsieh et al. (2013)