› 1z19

X-ray diffraction
2.8Å resolution

Crystal structure of a lambda integrase(75-356) dimer bound to a COC' core site

Released:
DOI: 10.2210/pdb1z19/pdb
PDB entry 1z19 coloured by chain, front view.
PDB entry 1z19 coloured by chain, side view.
PDB entry 1z19 coloured by chain, top view.
Source organism: Lambdavirus lambda
Primary publication:
A structural basis for allosteric control of DNA recombination by lambda integrase.
Biswas T, Aihara H, Radman-Livaja M, Filman D, Landy A, Ellenberger T
Nature 435 1059-66 (2005)
PMID: 15973401

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114876 (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
Integrase Chains: A, B
Molecule details ›
Chains: A, B
Length: 283 amino acids
Theoretical weight: 32.29 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli
UniProt:
  • Canonical: P03700 (Residues: 74-356; Coverage: 80%)
Gene name: int
Sequence domains:
Structure domains:
5'-D(*CP*TP*CP*GP*TP*TP*CP*AP*GP*CP*TP*TP*TP*TP*TP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 16 nucleotides
Theoretical weight: 4.82 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided
5'-D(P*TP*TP*TP*AP*TP*AP*CP*TP*AP*AP*GP*TP*TP*GP*GP*CP*AP*TP*TP*A)-3' Chain: D
Molecule details ›
Chain: D
Length: 20 nucleotides
Theoretical weight: 6.14 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided
33-MER Chain: E
Molecule details ›
Chain: E
Length: 33 nucleotides
Theoretical weight: 10.2 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided

Ligands and Environments

No bound ligands
2 modified residues:
Ligand MSE 16 x MSE
Ligand PTR 2 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21212
Unit cell:
a: 109.297Å b: 121.481Å c: 65.387Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.262
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

19 review citations

Mechanisms of site-specific recombination.
Grindley et al. (2006)
18 more

6 mentions without citation

DNA arms do the legwork to ensure the directionality of lambda site-specific recombination.
Radman-Livaja et al. (2006)
5 more