Function and Biology Details
Biochemical function:
Biological process:
Cellular component:
- not assigned
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136537 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Replication protein A 32 kDa subunit
Molecule details ›
Chain: A
Length: 103 amino acids
Theoretical weight: 11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Replication protein A C terminal
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Length: 103 amino acids
Theoretical weight: 11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P15927 (Residues: 172-270; Coverage: 37%)
Sequence domains: Replication protein A C terminal
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
Large T antigen
Molecule details ›
Chain: B
Length: 131 amino acids
Theoretical weight: 15.11 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
Structure domains: Replication Protein E1; Chain: A,
Length: 131 amino acids
Theoretical weight: 15.11 KDa
Source organism: Macaca mulatta polyomavirus 1
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P03070 (Residues: 131-259; Coverage: 18%)
Structure domains: Replication Protein E1; Chain: A,
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Refinement method:
Rigid body docking, Semi-flexible simulated annealing, Refinement using explicit water
Expression system: Escherichia coli BL21(DE3)
