› 1z2l

X-ray diffraction
2.25Å resolution

Crystal structure of Allantoate-amidohydrolase from E.coli K12 in complex with substrate Allantoate

Released:
DOI: 10.2210/pdb1z2l/pdb
PDB entry 1z2l coloured by chain, front view.
PDB entry 1z2l coloured by chain, side view.
PDB entry 1z2l coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Structural analysis of a ternary complex of allantoate amidohydrolase from Escherichia coli reveals its mechanics.
Agarwal R, Burley SK, Swaminathan S
J Mol Biol 368 450-63 (2007)
PMID: 17362992

Function and Biology Details

Reaction catalysed: 
Allantoate + H(2)O = (S)-ureidoglycine + NH(3) + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160166 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Allantoate amidohydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 423 amino acids
Theoretical weight: 47.1 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P77425 (Residues: 2-411; Coverage: 100%)
Gene names: JW0504, allC, b0516, glxB7, ylbB
Sequence domains: Peptidase family M20/M25/M40
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 4 x ZN
Ligand SO4 2 x SO4
Ligand 1AL 1 x 1AL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21212
Unit cell:
a: 95.532Å b: 186.579Å c: 49.218Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.229 0.273
Expression system: Escherichia coli

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Citations

2 review citations

The development of efflux pump inhibitors to treat Gram-negative infections.
Blanco et al. (2018)
1 more

5 mentions without citation

Ligands in PSI structures.
Kumar et al. (2010)
4 more