› 1z2o

X-ray diffraction
1.24Å resolution

Inositol 1,3,4-trisphosphate 5/6-Kinase in complex with mg2+/ADP/Ins(1,3,4,6)P4

Released:
DOI: 10.2210/pdb1z2o/pdb
PDB entry 1z2o coloured by chain, front view.
PDB entry 1z2o coloured by chain, side view.
PDB entry 1z2o coloured by chain, top view.
Source organism: Entamoeba histolytica HM-1:IMSS
Primary publication:
Specificity determinants in inositol polyphosphate synthesis: crystal structure of inositol 1,3,4-trisphosphate 5/6-kinase.
Miller GJ, Wilson MP, Majerus PW, Hurley JH
Mol Cell 18 201-12 (2005)
PMID: 15837423

Function and Biology Details

Reactions catalysed: 
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate = ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
ATP + 1D-myo-inositol 1,3,4-trisphosphate = ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195056 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inositol-tetrakisphosphate 1-kinase Chain: X
Molecule details ›
Chain: X
Length: 324 amino acids
Theoretical weight: 36.94 KDa
Source organism: Entamoeba histolytica HM-1:IMSS
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9XYQ1 (Residues: 1-319; Coverage: 100%)
Gene names: 151.t00008, ITPK1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ADP 1 x ADP
Ligand I4P 1 x I4P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1, null
Spacegroup: P21
Unit cell:
a: 38.189Å b: 94.294Å c: 47.351Å
α: 90° β: 110.65° γ: 90°
R-values:
R R work R free
0.189 0.189 0.208
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
13 more

2 mentions without citation

X-ray structures of magnesium and manganese complexes with the N-terminal domain of calmodulin: insights into the mechanism and specificity of metal ion binding to an EF-hand.
Senguen et al. (2012)
1 more