› 1z35

X-ray diffraction
2.5Å resolution

Crystal structure of Trichomonas vaginalis purine nucleoside phosphorylase complexed with 2-fluoroadenosine

Released:
DOI: 10.2210/pdb1z35/pdb
PDB entry 1z35 coloured by chain, front view.
PDB entry 1z35 coloured by chain, side view.
PDB entry 1z35 coloured by chain, top view.
Source organism: Trichomonas vaginalis
Primary publication:
Identification of a subversive substrate of Trichomonas vaginalis purine nucleoside phosphorylase and the crystal structure of the enzyme-substrate complex.
Zang Y, Wang WH, Wu SW, Ealick SE, Wang CC
J Biol Chem 280 22318-25 (2005)
PMID: 15817485

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162588 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
purine nucleoside phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 235 amino acids
Theoretical weight: 25.81 KDa
Source organism: Trichomonas vaginalis
Expression system: Escherichia coli
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

1 bound ligand:
Ligand 2FA 1 x 2FA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P4132
Unit cell:
a: 136.8Å b: 136.8Å c: 136.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.244
Expression system: Escherichia coli

Quick links

Citations

9 citation in other articles

Susceptibility in vitro of clinically metronidazole-resistant Trichomonas vaginalis to nitazoxanide, toyocamycin, and 2-fluoro-2'-deoxyadenosine.
Wright et al. (2010)
8 more

2 mentions without citation

Inhibition and structure of Trichomonas vaginalis purine nucleoside phosphorylase with picomolar transition state analogues.
Rinaldo-Matthis et al. (2007)
1 more