› 1z3e

X-ray diffraction
1.5Å resolution

Crystal Structure of Spx in Complex with the C-terminal Domain of the RNA Polymerase Alpha Subunit

Released:
DOI: 10.2210/pdb1z3e/pdb
Model geometry
Fit model/data
PDB entry 1z3e coloured by chain, front view.
PDB entry 1z3e coloured by chain, side view.
PDB entry 1z3e coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of the Bacillus subtilis anti-alpha, global transcriptional regulator, Spx, in complex with the alpha C-terminal domain of RNA polymerase.
Newberry KJ, Nakano S, Zuber P, Brennan RG
Proc Natl Acad Sci U S A 102 15839-44 (2005)
PMID: 16249335

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128311 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Global transcriptional regulator Spx Chain: A
Molecule details ›
Chain: A
Length: 132 amino acids
Theoretical weight: 15.69 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O31602 (Residues: 1-131; Coverage: 100%)
Gene names: BSU11500, spx, spxA, yjbD
Sequence domains: ArsC family
Structure domains: Glutaredoxin
DNA-directed RNA polymerase subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 73 amino acids
Theoretical weight: 8.38 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P20429 (Residues: 245-314; Coverage: 22%)
Gene names: BSU01430, rpoA
Sequence domains: Bacterial RNA polymerase, alpha chain C terminal domain
Structure domains: 5' to 3' exonuclease, C-terminal subdomain

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: R3
Unit cell:
a: 96.44Å b: 96.44Å c: 57.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.196 0.22
Expression system: Escherichia coli BL21

Quick links

Citations

14 review citations

Local and global regulation of transcription initiation in bacteria.
Browning et al. (2016)
13 more

8 mentions without citation

Structural biology of bacterial RNA polymerase.
Murakami KS. (2015)
7 more