› 1z68

X-ray diffraction
2.6Å resolution

Crystal Structure Of Human Fibroblast Activation Protein alpha

Released:
DOI: 10.2210/pdb1z68/pdb
PDB entry 1z68 coloured by chain, front view.
PDB entry 1z68 coloured by chain, side view.
PDB entry 1z68 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and kinetic analysis of the substrate specificity of human fibroblast activation protein alpha.
Aertgeerts K, Levin I, Shi L, Snell GP, Jennings A, Prasad GS, Zhang Y, Kraus ML, Salakian S, Sridhar V, Wijnands R, Tennant MG
J Biol Chem 280 19441-4 (2005)
PMID: 15809306

Function and Biology Details

Reactions catalysed: 
Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, from a polypeptide, preferentially when Yaa is Pro, provided Zaa is neither Pro nor hydroxyproline.
Hydrolysis of --Pro-|- and to a lesser extent --Ala-|- in oligopeptides.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Prolyl endopeptidase FAP Chains: A, B
Molecule details ›
Chains: A, B
Length: 719 amino acids
Theoretical weight: 83.28 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q12884 (Residues: 39-757; Coverage: 95%)
Gene name: FAP
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P212121
Unit cell:
a: 70.295Å b: 152.58Å c: 214.855Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.223 0.283
Expression system: Spodoptera frugiperda

Quick links

Citations

26 review citations

The metabolic serine hydrolases and their functions in mammalian physiology and disease.
Long et al. (2011)
25 more

19 mentions without citation

The role of fibroblast activation protein in health and malignancy.
Fitzgerald et al. (2020)
18 more