› 1z6k

X-ray diffraction
2.3Å resolution

Citrate lyase beta subunit complexed with oxaloacetate and magnesium from M. tuberculosis

Released:
DOI: 10.2210/pdb1z6k/pdb
PDB entry 1z6k coloured by chain, front view.
PDB entry 1z6k coloured by chain, side view.
PDB entry 1z6k coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
The structure and computational analysis of Mycobacterium tuberculosis protein CitE suggest a novel enzymatic function.
Goulding CW, Bowers PM, Segelke B, Lekin T, Kim CY, Terwilliger TC, Eisenberg D
J Mol Biol 365 275-83 (2007)
PMID: 17064730

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161969 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Citrate lyase subunit beta-like protein Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 31.42 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WPE1 (Residues: 1-273; Coverage: 100%)
Gene names: Rv2498c, citE
Sequence domains: HpcH/HpaI aldolase/citrate lyase family
Structure domains: Phosphoenolpyruvate-binding domains

Ligands and Environments

2 bound ligands:
Ligand OAA 1 x OAA
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: R32
Unit cell:
a: 91.28Å b: 91.28Å c: 219.263Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.256 0.224 0.256
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Metabolomic strategies for the identification of new enzyme functions and metabolic pathways.
Prosser et al. (2014)
2 more

7 mentions without citation

The Human Knockout Gene CLYBL Connects Itaconate to Vitamin B12.
Shen et al. (2017)
6 more