› 1z75

X-ray diffraction
2.4Å resolution

Crystal Structure of ArnA dehydrogenase (decarboxylase) domain, R619M mutant

Released:
DOI: 10.2210/pdb1z75/pdb
Model geometry
Fit model/data
PDB entry 1z75 coloured by chain, front view.
PDB entry 1z75 coloured by chain, side view.
PDB entry 1z75 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure and mechanism of ArnA: conformational change implies ordered dehydrogenase mechanism in key enzyme for polymyxin resistance.
Gatzeva-Topalova PZ, May AP, Sousa MC
Structure 13 929-42 (2005)
PMID: 15939024

Function and Biology Details

Reactions catalysed: 
UDP-alpha-D-glucuronate + NAD(+) = UDP-beta-L-threo-pentopyranos-4-ulose+ CO2 + NADH.
UDP-4-amino-4-deoxy-beta-L-arabinose + (6R)-10-formyltetrahydrofolate =UDP-4-deoxy-4-formamido-beta-L-arabinose + (6S)-5,6,7,8-tetrahydrofolate+ H(+).
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-160160 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional polymyxin resistance protein ArnA Chain: A
Molecule details ›
Chain: A
Length: 358 amino acids
Theoretical weight: 41.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P77398 (Residues: 306-660; Coverage: 54%)
Gene names: JW2249, arnA, b2255, pmrI, yfbG
Sequence domains: NAD dependent epimerase/dehydratase family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 2 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU
Spacegroup: P4132
Unit cell:
a: 150.364Å b: 150.364Å c: 150.364Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.203 0.231
Expression system: Escherichia coli

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Citations

8 review citations

Lipid A modification systems in gram-negative bacteria.
Raetz et al. (2007)
7 more

2 mentions without citation

Structure and mechanism of ArnA: conformational change implies ordered dehydrogenase mechanism in key enzyme for polymyxin resistance.
Gatzeva-Topalova et al. (2005)
1 more