› 1z7y

X-ray diffraction
2.7Å resolution

Crystal Structure of the Arabidopsis thaliana O-Acetylserine Sulfhydrylase K46A mutant

Released:
DOI: 10.2210/pdb1z7y/pdb
PDB entry 1z7y coloured by chain, front view.
PDB entry 1z7y coloured by chain, side view.
PDB entry 1z7y coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Molecular basis of cysteine biosynthesis in plants: structural and functional analysis of O-acetylserine sulfhydrylase from Arabidopsis thaliana.
Bonner ER, Cahoon RE, Knapke SM, Jez JM
J Biol Chem 280 38803-13 (2005)
PMID: 16166087

Function and Biology Details

Reaction catalysed: 
O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-155688 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine synthase 1 Chain: A
Molecule details ›
Chain: A
Length: 322 amino acids
Theoretical weight: 33.78 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P47998 (Residues: 1-322; Coverage: 100%)
Gene names: At4g14880, OAS1, OASA1, OASS, OLD3, dl3480c
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand AA5 1 x AA5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: P43212
Unit cell:
a: 105.856Å b: 105.856Å c: 100.159Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.251 0.277
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Cysteine and cysteine-related signaling pathways in Arabidopsis thaliana.
Romero et al. (2014)
14 more

3 mentions without citation

Insights into multifaceted activities of CysK for therapeutic interventions.
Joshi et al. (2019)
2 more