› 1z85

X-ray diffraction
2.12Å resolution

Crystal structure of a predicted rna methyltransferase (tm1380) from thermotoga maritima msb8 at 2.12 A resolution

Released:
DOI: 10.2210/pdb1z85/pdb
PDB entry 1z85 coloured by chain, front view.
PDB entry 1z85 coloured by chain, side view.
PDB entry 1z85 coloured by chain, top view.
Source organism: Thermotoga maritima MSB8
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + uracil(1498) in 16S rRNA = S-adenosyl-L-homocysteine + N(3)-methyluracil(1498) in 16S rRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194775 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase E Chains: A, B
Molecule details ›
Chains: A, B
Length: 234 amino acids
Theoretical weight: 26.74 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9X1A0 (Residues: 1-222; Coverage: 100%)
Gene name: TM_1380
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 6 x CL
Ligand EDO 1 x EDO
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P212121
Unit cell:
a: 65.652Å b: 82.473Å c: 106.264Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.174 0.171 0.222
Expression system: Escherichia coli

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