› 1z8h

X-ray diffraction
2.02Å resolution

CRYSTAL STRUCTURE OF a GDSL-like lipase (ALR1529) FROM NOSTOC SP. PCC 7120 AT 2.02 A RESOLUTION

Released:
DOI: 10.2210/pdb1z8h/pdb
PDB entry 1z8h coloured by chain, front view.
PDB entry 1z8h coloured by chain, side view.
PDB entry 1z8h coloured by chain, top view.
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187133 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SGNH hydrolase-type esterase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 218 amino acids
Theoretical weight: 25.23 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8YWS4 (Residues: 1-206; Coverage: 100%)
Gene name: alr1529
Sequence domains: GDSL-like Lipase/Acylhydrolase family
Structure domains: SGNH hydrolase

Ligands and Environments

2 bound ligands:
Ligand UNL 4 x UNL
Ligand IPA 3 x IPA
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 64.504Å b: 69.806Å c: 211.028Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.155 0.195
Expression system: Escherichia coli

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