› 1z8r

Solution NMR

2A cysteine proteinase from human coxsackievirus B4 (strain JVB / Benschoten / New York / 51)

Released:
DOI: 10.2210/pdb1z8r/pdb
PDB entry 1z8r coloured by chain, ensemble of 17 models, front view.
PDB entry 1z8r coloured by chain, ensemble of 17 models, side view.
PDB entry 1z8r coloured by chain, ensemble of 17 models, top view.
Source organism: Coxsackievirus B4
Primary publication:
Structure and dynamics of coxsackievirus B4 2A proteinase, an enyzme involved in the etiology of heart disease.
Baxter NJ, Roetzer A, Liebig HD, Sedelnikova SE, Hounslow AM, Skern T, Waltho JP
J Virol 80 1451-62 (2006)
PMID: 16415022

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140086 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protease 2A Chain: A
Molecule details ›
Chain: A
Length: 166 amino acids
Theoretical weight: 18.43 KDa
Source organism: Coxsackievirus B4
Expression system: Escherichia coli
UniProt:
  • Canonical: P08292 (Residues: 842-999; Coverage: 7%)
Sequence domains: Picornavirus core protein 2A
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics followed by cartesian slow-cool annealing energy minimisation
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Selective inhibitors of picornavirus replication.
De Palma et al. (2008)
5 more

11 mentions without citation

Structures and Corresponding Functions of Five Types of Picornaviral 2A Proteins.
Yang et al. (2017)
10 more