› 1z98

X-ray diffraction
2.1Å resolution

Crystal structure of the spinach aquaporin SoPIP2;1 in a closed conformation

Released:
DOI: 10.2210/pdb1z98/pdb
PDB entry 1z98 coloured by chain, front view.
PDB entry 1z98 coloured by chain, side view.
PDB entry 1z98 coloured by chain, top view.
Source organism: Spinacia oleracea
Primary publication:
Structural mechanism of plant aquaporin gating.
T��rnroth-Horsefield S, Wang Y, Hedfalk K, Johanson U, Karlsson M, Tajkhorshid E, Neutze R, Kjellbom P
Nature 439 688-94 (2006)
PMID: 16340961

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174946 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aquaporin PIP-type Chains: A, M
Molecule details ›
Chains: A, M
Length: 281 amino acids
Theoretical weight: 29.93 KDa
Source organism: Spinacia oleracea
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q41372 (Residues: 1-281; Coverage: 100%)
Gene name: LOC110800173
Sequence domains: Major intrinsic protein
Structure domains: Glycerol uptake facilitator protein.

Ligands and Environments

1 bound ligand:
Ligand CD 2 x CD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I4
Unit cell:
a: 90.097Å b: 90.097Å c: 189.181Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.208
Expression system: Komagataella pastoris

Quick links

Citations

103 review citations

Plant aquaporins: membrane channels with multiple integrated functions.
Maurel et al. (2008)
102 more

36 mentions without citation

The recombinant expression systems for structure determination of eukaryotic membrane proteins.
He et al. (2014)
35 more