› 1z9c

X-ray diffraction
2.64Å resolution

Crystal structure of OhrR bound to the ohrA promoter: Structure of MarR family protein with operator DNA

Released:
DOI: 10.2210/pdb1z9c/pdb
PDB entry 1z9c coloured by chain, front view.
PDB entry 1z9c coloured by chain, side view.
PDB entry 1z9c coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Structure of an OhrR-ohrA operator complex reveals the DNA binding mechanism of the MarR family.
Hong M, Fuangthong M, Helmann JD, Brennan RG
Mol Cell 20 131-41 (2005)
PMID: 16209951

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114885 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Organic hydroperoxide resistance transcriptional regulator Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 147 amino acids
Theoretical weight: 17.01 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O34777 (Residues: 1-147; Coverage: 100%)
Gene names: BSU13150, ohrR, ykmA
Sequence domains: MarR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (29-MER) Chains: G, I, K
Molecule details ›
Chains: G, I, K
Length: 29 nucleotides
Theoretical weight: 8.9 KDa
Source organism: Bacillus subtilis
Expression system: Not provided
DNA (29-MER) Chains: H, J, L
Molecule details ›
Chains: H, J, L
Length: 29 nucleotides
Theoretical weight: 8.91 KDa
Source organism: Bacillus subtilis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 81.899Å b: 80.96Å c: 109.34Å
α: 90° β: 100.2° γ: 90°
R-values:
R R work R free
0.216 0.216 0.277
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

19 review citations

ROS as signalling molecules: mechanisms that generate specificity in ROS homeostasis.
D'Autréaux et al. (2007)
18 more

31 mentions without citation

Coordination chemistry of bacterial metal transport and sensing.
Ma et al. (2009)
30 more