› 1z9t

X-ray diffraction
1.54Å resolution

CRYSTAL STRUCTURE OF A PUTATIVE LACCASE (YFIH) FROM ESCHERICHIA COLI AT 1.54 A RESOLUTION

Released:
DOI: 10.2210/pdb1z9t/pdb
PDB entry 1z9t coloured by chain, front view.
PDB entry 1z9t coloured by chain, side view.
PDB entry 1z9t coloured by chain, top view.
Source organism: Escherichia coli
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reactions catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
2'-deoxyadenosine + H(2)O = 2'-deoxyinosine + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152626 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase YfiH Chain: A
Molecule details ›
Chain: A
Length: 255 amino acids
Theoretical weight: 28.06 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P33644 (Residues: 1-243; Coverage: 100%)
Gene names: JW2575, b2593, yfiH
Sequence domains: Multi-copper polyphenol oxidoreductase laccase
Structure domains: CNF1/YfiH-like putative cysteine hydrolases

Ligands and Environments

2 bound ligands:
Ligand CIT 2 x CIT
Ligand EDO 19 x EDO
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P43212
Unit cell:
a: 80.711Å b: 80.711Å c: 90.589Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.143 0.142 0.172
Expression system: Escherichia coli

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