1zbe

X-ray diffraction
3Å resolution

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero 240-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136759 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Capsid protein VP1 Chain: 1
Molecule details ›
Chain: 1
Length: 212 amino acids
Theoretical weight: 23.29 KDa
Source organism: Foot-and-mouth disease virus
UniProt:
  • Canonical: P03306 (Residues: 726-937; Coverage: 9%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP2 Chain: 2
Molecule details ›
Chain: 2
Length: 218 amino acids
Theoretical weight: 24.68 KDa
Source organism: Foot-and-mouth disease virus
UniProt:
  • Canonical: P03306 (Residues: 287-504; Coverage: 9%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP3 Chain: 3
Molecule details ›
Chain: 3
Length: 221 amino acids
Theoretical weight: 24.23 KDa
Source organism: Foot-and-mouth disease virus
UniProt:
  • Canonical: P03306 (Residues: 505-725; Coverage: 10%)
Sequence domains: picornavirus capsid protein
Structure domains: Jelly Rolls
Capsid protein VP4 Chain: 4
Molecule details ›
Chain: 4
Length: 85 amino acids
Theoretical weight: 8.79 KDa
Source organism: Foot-and-mouth disease virus
UniProt:
  • Canonical: P03306 (Residues: 202-286; Coverage: 4%)
Sequence domains: Viral protein VP4 subunit
Structure domains: Capsid protein VP4 superfamily, Picornavirus

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: R3
Unit cell:
a: 306.2Å b: 306.2Å c: 712.8Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.214 not available not available