› 1zbh

X-ray diffraction
3Å resolution

3'-end specific recognition of histone mRNA stem-loop by 3'-exonuclease

Released:
DOI: 10.2210/pdb1zbh/pdb
PDB entry 1zbh coloured by chain, front view.
PDB entry 1zbh coloured by chain, side view.
PDB entry 1zbh coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Cheng Y, Patel DJ

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184864 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
3'-5' exoribonuclease 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 299 amino acids
Theoretical weight: 34.66 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8IV48 (Residues: 51-349; Coverage: 86%)
Gene names: 3'EXO, ERI1, THEX1
Sequence domains:
Structure domains:
5'-R(*CP*CP*GP*GP*CP*UP*CP*UP*UP*UP*UP*CP*AP*GP*AP*GP*CP*CP*GP*G)-3' Chains: E, F
Molecule details ›
Chains: E, F
Length: 20 nucleotides
Theoretical weight: 6.35 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MG 8 x MG
Ligand AMP 4 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 50.386Å b: 195.148Å c: 87.966Å
α: 90° β: 92.13° γ: 90°
R-values:
R R work R free
0.214 0.209 0.26
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

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