› 1zca

X-ray diffraction
2.9Å resolution

Crystal structure of G alpha 12 in complex with GDP, Mg2+ and AlF4-

Released:
DOI: 10.2210/pdb1zca/pdb
PDB entry 1zca coloured by chain, front view.
PDB entry 1zca coloured by chain, side view.
PDB entry 1zca coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
A new approach to producing functional G alpha subunits yields the activated and deactivated structures of G alpha(12/13) proteins.
Kreutz B, Yau DM, Nance MR, Tanabe S, Tesmer JJ, Kozasa T
Biochemistry 45 167-74 (2006)
PMID: 16388592

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151038 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein subunit alpha-12 Chains: A, B
Molecule details ›
Chains: A, B
Length: 359 amino acids
Theoretical weight: 41.95 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P27600 (Residues: 25-31, 32-47, 48-379; Coverage: 94%)
Gene names: Gna-12, Gna12
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ALF 2 x ALF
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 57.501Å b: 85.23Å c: 82.885Å
α: 90° β: 106.09° γ: 90°
R-values:
R R work R free
0.239 0.236 0.296
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Regulation and physiological functions of G12/13-mediated signaling pathways.
Suzuki et al. (2009)
3 more

13 mentions without citation

Discovery of new GPCR biology: one receptor structure at a time.
Hanson et al. (2009)
12 more