› 1zdr

X-ray diffraction
2Å resolution

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 164 amino acids
Theoretical weight: 18.72 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5KZ26 (Residues: 1-164; Coverage: 100%)
Gene name: GK1775
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P4212
Unit cell:
a: 104.65Å b: 104.65Å c: 116.37Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.21 0.248
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Hydrogen tunneling links protein dynamics to enzyme catalysis.
Klinman et al. (2013)
2 more

16 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
15 more