› 1ze2

X-ray diffraction
3Å resolution

Conformational change of pseudouridine 55 synthase upon its association with RNA substrate

Released:
DOI: 10.2210/pdb1ze2/pdb
PDB entry 1ze2 coloured by chain, front view.
PDB entry 1ze2 coloured by chain, side view.
PDB entry 1ze2 coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Conformational change of pseudouridine 55 synthase upon its association with RNA substrate.
Phannachet K, Huang RH
Nucleic Acids Res 32 1422-9 (2004)
PMID: 14990747

Function and Biology Details

Reaction catalysed: 
tRNA uridine(55) = tRNA pseudouridine(55)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194646 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
tRNA pseudouridine synthase B Chains: A, B
Molecule details ›
Chains: A, B
Length: 309 amino acids
Theoretical weight: 35.54 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9WZW0 (Residues: 1-309; Coverage: 100%)
Gene names: TM_0856, truB
Sequence domains:
Structure domains:
5'-R(*GP*GP*CP*CP*AP*CP*GP*GP*UP*(FHU)P*CP*GP*AP*AP*UP*CP*CP*GP*UP*GP*GP*C)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 22 nucleotides
Theoretical weight: 7.1 KDa
Source organism: Thermotoga maritima
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand FHU 2 x FHU

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C, APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 134.975Å b: 134.975Å c: 139.537Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.261 0.318
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

9 review citations

Pseudouridine synthases.
Hamma et al. (2006)
8 more

3 mentions without citation

Conformational changes in redox pairs of protein structures.
Fan et al. (2009)
2 more