› 1zej

X-ray diffraction
2Å resolution

Crystal structure of the 3-hydroxyacyl-coa dehydrogenase (hbd-9, af2017) from archaeoglobus fulgidus dsm 4304 at 2.00 A resolution

Released:
DOI: 10.2210/pdb1zej/pdb
PDB entry 1zej coloured by chain, front view.
PDB entry 1zej coloured by chain, side view.
PDB entry 1zej coloured by chain, top view.
Source organism: Archaeoglobus fulgidus DSM 4304
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
L-gulonate + NAD(+) = 3-dehydro-L-gulonate + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127999 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-hydroxyacyl-CoA dehydrogenase (Hbd-9) Chain: A
Molecule details ›
Chain: A
Length: 293 amino acids
Theoretical weight: 33.54 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli
UniProt:
  • Canonical: O28262 (Residues: 1-281; Coverage: 100%)
Gene name: AF_2017
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand PE8 1 x PE8
1 modified residue:
Ligand MSE 7 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P622
Unit cell:
a: 146.331Å b: 146.331Å c: 62.73Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.181 0.179 0.221
Expression system: Escherichia coli

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