› 1zel

X-ray diffraction
1.93Å resolution

Crystal structure of RV2827C protein from Mycobacterium tuberculosis

Released:
DOI: 10.2210/pdb1zel/pdb
PDB entry 1zel coloured by chain, front view.
PDB entry 1zel coloured by chain, side view.
PDB entry 1zel coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Structural analysis reveals DNA binding properties of Rv2827c, a hypothetical protein from Mycobacterium tuberculosis.
Janowski R, Panjikar S, Eddine AN, Kaufmann SH, Weiss MS
J Struct Funct Genomics 10 137-50 (2009)
PMID: 19184528

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181472 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AbiEi antitoxin C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 298 amino acids
Theoretical weight: 32.61 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7D6H9 (Residues: 21-315; Coverage: 94%)
Gene name: MT2894
Sequence domains: AbiEi antitoxin C-terminal domain
Structure domains: replication protein C, winged helix domain

Ligands and Environments

4 bound ligands:
Ligand NA 2 x NA
Ligand MPD 1 x MPD
Ligand FMT 15 x FMT
Ligand ACT 2 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13, EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P21212
Unit cell:
a: 87.419Å b: 180.653Å c: 35.112Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.225
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Normal Mode Analysis as a Routine Part of a Structural Investigation.
Bauer et al. (2019)
4 other articles

4 mentions without citation

Characterization of a toxin-antitoxin system in Mycobacterium tuberculosis suggests neutralization by phosphorylation as the antitoxicity mechanism.
Yu et al. (2020)
3 more