› 1zg1

X-ray diffraction
2.3Å resolution

NarL complexed to nirB promoter non-palindromic tail-to-tail DNA site

Released:
DOI: 10.2210/pdb1zg1/pdb
PDB entry 1zg1 coloured by chain, front view.
PDB entry 1zg1 coloured by chain, side view.
PDB entry 1zg1 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Primary and secondary modes of DNA recognition by the NarL two-component response regulator.
Maris AE, Kaczor-Grzeskowiak M, Ma Z, Kopka ML, Gunsalus RP, Dickerson RE
Biochemistry 44 14538-52 (2005)
PMID: 16262254

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114914 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Nitrate/nitrite response regulator protein NarL Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 82 amino acids
Theoretical weight: 9.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AF28 (Residues: 147-216; Coverage: 32%)
Gene names: JW1212, b1221, frdR, narL
Sequence domains: Bacterial regulatory proteins, luxR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*CP*GP*TP*AP*CP*TP*CP*CP*TP*TP*AP*AP*TP*GP*GP*GP*TP*AP*CP*G)-3' Chains: C, H
Molecule details ›
Chains: C, H
Length: 20 nucleotides
Theoretical weight: 6.12 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*CP*GP*TP*AP*CP*CP*CP*AP*TP*TP*AP*AP*GP*GP*AP*GP*TP*AP*CP*G)-3' Chains: D, G
Molecule details ›
Chains: D, G
Length: 20 nucleotides
Theoretical weight: 6.14 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 9 x SO4
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 76.58Å b: 52.82Å c: 84.97Å
α: 90° β: 90.07° γ: 90°
R-values:
R R work R free
0.257 0.257 0.286
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Signal transduction and adaptive regulation through bacterial two-component systems: the Escherichia coli AtoSC paradigm.
Kyriakidis et al. (2009)
20 other articles

2 mentions without citation

The molecular basis of TCR germline bias for MHC is surprisingly simple.
Garcia et al. (2009)
1 more