› 1zi7

X-ray diffraction
2.5Å resolution

Structure of truncated yeast oxysterol binding protein Osh4

Released:
DOI: 10.2210/pdb1zi7/pdb
PDB entry 1zi7 coloured by chain, front view.
PDB entry 1zi7 coloured by chain, side view.
PDB entry 1zi7 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural mechanism for sterol sensing and transport by OSBP-related proteins.
Im YJ, Raychaudhuri S, Prinz WA, Hurley JH
Nature 437 154-8 (2005)
PMID: 16136145

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-153044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Oxysterol-binding protein homolog 4 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 406 amino acids
Theoretical weight: 46.49 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P35844 (Residues: 30-434; Coverage: 93%)
Gene names: BSR3, KES1, LPI3C, OSH4, P2614, YPL145C
Sequence domains: Oxysterol-binding protein
Structure domains: Helix Hairpins

Ligands and Environments

1 bound ligand:
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21
Unit cell:
a: 93.903Å b: 100.853Å c: 117.624Å
α: 90° β: 91.53° γ: 90°
R-values:
R R work R free
0.213 0.21 0.246
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

89 review citations

Structure and function of ER membrane contact sites with other organelles.
Phillips et al. (2016)
88 more

3 mentions without citation

Polyphosphoinositide-Binding Domains: Insights from Peripheral Membrane and Lipid-Transfer Proteins.
Pemberton et al. (2019)
2 more