› 1zkp

X-ray diffraction
1.5Å resolution

1.5A Resolution Crystal Structure of a Metallo Beta Lactamase Family Protein, the ELAC Homolgue of Bacillus anthracis, a Putative Ribonuclease

Released:
DOI: 10.2210/pdb1zkp/pdb
PDB entry 1zkp coloured by chain, front view.
PDB entry 1zkp coloured by chain, side view.
PDB entry 1zkp coloured by chain, top view.
Source organism: Bacillus anthracis str. Ames
Entry authors: Brunzelle JS, Minasov G, Shuvalova L, Collart FR, Anderson WF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105756 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 268 amino acids
Theoretical weight: 29.83 KDa
Source organism: Bacillus anthracis str. Ames
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A6L8PRK4 (Residues: 1-244; Coverage: 100%)
Gene name: GBAA_1088
Sequence domains: Beta-lactamase superfamily domain
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

3 bound ligands:
Ligand ZN 8 x ZN
Ligand NA 4 x NA
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 24 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID, APS BEAMLINE 5ID-B
Spacegroup: P21
Unit cell:
a: 73.375Å b: 91.19Å c: 78.466Å
α: 90° β: 115.31° γ: 90°
R-values:
R R work R free
0.149 0.147 0.18
Expression system: Escherichia coli BL21(DE3)

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