› 1zlk

X-ray diffraction
3.1Å resolution

Crystal Structure of the Mycobacterium tuberculosis Hypoxic Response Regulator DosR C-terminal Domain-DNA Complex

Released:
DOI: 10.2210/pdb1zlk/pdb
PDB entry 1zlk coloured by chain, front view.
PDB entry 1zlk coloured by chain, side view.
PDB entry 1zlk coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structures of Mycobacterium tuberculosis DosR and DosR-DNA complex involved in gene activation during adaptation to hypoxic latency.
Wisedchaisri G, Wu M, Rice AE, Roberts DM, Sherman DR, Hol WG
J Mol Biol 354 630-41 (2005)
PMID: 16246368

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114924 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-binding transcriptional activator DevR/DosR Chains: A, B
Molecule details ›
Chains: A, B
Length: 95 amino acids
Theoretical weight: 10.51 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WMF9 (Residues: 144-217; Coverage: 34%)
Gene names: Rv3133c, devR, dosR
Sequence domains: Bacterial regulatory proteins, luxR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*GP*GP*CP*CP*CP*GP*CP*GP*CP*TP*TP*TP*GP*GP*GP*GP*AP*CP*TP*AP*AP*AP*GP*TP*CP*CP*CP*TP*AP*AP*CP*CP*CP*TP*GP*GP*CP*CP*AP*CP*GP*AP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 43 nucleotides
Theoretical weight: 13.18 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Not provided
5'-D(*CP*GP*TP*GP*GP*CP*CP*AP*GP*GP*GP*TP*TP*AP*GP*GP*GP*AP*CP*TP*TP*TP*AP*GP*TP*CP*CP*CP*CP*AP*AP*AP*GP*CP*GP*CP*GP*GP*GP*CP*CP*AP*T)-3' Chain: D
Molecule details ›
Chain: D
Length: 43 nucleotides
Theoretical weight: 13.3 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 142.397Å b: 58.791Å c: 82.933Å
α: 90° β: 125.5° γ: 90°
R-values:
R R work R free
0.272 0.272 0.288
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

3 review citations

Hypoxia: a window into Mycobacterium tuberculosis latency.
Rustad et al. (2009)
2 more

6 mentions without citation

Conservation of the three-dimensional structure in non-homologous or unrelated proteins.
Sousounis et al. (2012)
5 more