› 1zm8

X-ray diffraction
1.9Å resolution

Apo Crystal structure of Nuclease A from Anabaena sp.

Released:
DOI: 10.2210/pdb1zm8/pdb
PDB entry 1zm8 coloured by chain, front view.
PDB entry 1zm8 coloured by chain, side view.
PDB entry 1zm8 coloured by chain, top view.
Source organism: Anabaena sp.
Primary publication:
Structural insights into the mechanism of nuclease A, a betabeta alpha metal nuclease from Anabaena.
Ghosh M, Meiss G, Pingoud A, London RE, Pedersen LC
J Biol Chem 280 27990-7 (2005)
PMID: 15897201

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153653 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nuclease Chain: A
Molecule details ›
Chain: A
Length: 259 amino acids
Theoretical weight: 28.54 KDa
Source organism: Anabaena sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P38446 (Residues: 24-274; Coverage: 100%)
Gene names: all7362, nucA
Sequence domains: DNA/RNA non-specific endonuclease
Structure domains: Extracellular Endonuclease, subunit A

Ligands and Environments

3 bound ligands:
Ligand MN 3 x MN
Ligand SO4 2 x SO4
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 46.69Å b: 66.682Å c: 81.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.212 0.256
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Roles of metal ions in nucleases.
Dupureur CM. (2008)
1 more

11 mentions without citation

Lessons from making the Structural Classification of Proteins (SCOP) and their implications for protein structure modelling.
Andreeva A. (2016)
10 more