› 1zmy

X-ray diffraction
3Å resolution

cAbBCII-10 VHH framework with CDR loops of cAbLys3 grafted on it and in complex with hen egg white lysozyme

Released:
DOI: 10.2210/pdb1zmy/pdb
PDB entry 1zmy coloured by chain, front view.
PDB entry 1zmy coloured by chain, side view.
PDB entry 1zmy coloured by chain, top view.
Source organisms:
Primary publication:
Identification of a universal VHH framework to graft non-canonical antigen-binding loops of camel single-domain antibodies.
Saerens D, Pellis M, Loris R, Pardon E, Dumoulin M, Matagne A, Wyns L, Muyldermans S, Conrath K
J Mol Biol 352 597-607 (2005)
PMID: 16095608

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-208255 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Antibody cabbcII-10:lys3 Chain: A
Molecule details ›
Chain: A
Length: 142 amino acids
Theoretical weight: 15.21 KDa
Source organism: Camelus dromedarius
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Lysozyme C Chains: L, M
Molecule details ›
Chains: L, M
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P65
Unit cell:
a: 132.331Å b: 132.331Å c: 63.397Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 0.254
Expression system: Escherichia coli

Quick links

Citations

24 review citations

Nanobodies: natural single-domain antibodies.
Muyldermans S. (2013)
23 more

2 mentions without citation

Analysis and modeling of the variable region of camelid single-domain antibodies.
Sircar et al. (2011)
1 more