› 1zoi

X-ray diffraction
1.6Å resolution

Crystal Structure of a Stereoselective Esterase from Pseudomonas putida IFO12996

Released:
DOI: 10.2210/pdb1zoi/pdb
PDB entry 1zoi coloured by chain, front view.
PDB entry 1zoi coloured by chain, side view.
PDB entry 1zoi coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Stereoselective esterase from Pseudomonas putida IFO12996 reveals alpha/beta hydrolase folds for D-beta-acetylthioisobutyric acid synthesis.
Elmi F, Lee HT, Huang JY, Hsieh YC, Wang YL, Chen YJ, Shaw SY, Chen CJ
J Bacteriol 187 8470-6 (2005)
PMID: 16321951

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174566 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AB hydrolase-1 domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 276 amino acids
Theoretical weight: 30.2 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli
UniProt:
  • Canonical: Q3HWU8 (Residues: 1-276; Coverage: 100%)
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: P212121
Unit cell:
a: 50.556Å b: 98.108Å c: 153.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.22 0.239
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Esterases as stereoselective biocatalysts.
Romano et al. (2015)
13 other articles

13 mentions without citation

Crystal structure of the human N-Myc downstream-regulated gene 2 protein provides insight into its role as a tumor suppressor.
Hwang et al. (2011)
12 more