› 1zol

X-ray diffraction
1.9Å resolution

native beta-PGM

Released:
DOI: 10.2210/pdb1zol/pdb
PDB entry 1zol coloured by chain, front view.
PDB entry 1zol coloured by chain, side view.
PDB entry 1zol coloured by chain, top view.
Source organism: Lactococcus lactis
Primary publication:
Catalytic cycling in beta-phosphoglucomutase: a kinetic and structural analysis.
Zhang G, Dai J, Wang L, Dunaway-Mariano D, Tremblay LW, Allen KN
Biochemistry 44 9404-16 (2005)
PMID: 15996095

Function and Biology Details

Reaction catalysed: 
Beta-D-glucose 1-phosphate = beta-D-glucose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-phosphoglucomutase Chain: A
Molecule details ›
Chain: A
Length: 221 amino acids
Theoretical weight: 24.24 KDa
Source organism: Lactococcus lactis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P71447 (Residues: 1-221; Coverage: 100%)
Gene names: L0001, LL0429, pgmB
Sequence domains: haloacid dehalogenase-like hydrolase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 52.76Å b: 57.19Å c: 75.64Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.202 0.202 0.211
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Enzyme promiscuity: engine of evolutionary innovation.
Pandya et al. (2014)
23 other articles

6 mentions without citation

Panoramic view of a superfamily of phosphatases through substrate profiling.
Huang et al. (2015)
5 more