› 1zt1

X-ray diffraction
2.5Å resolution

crystal structure of class I MHC H-2Kk in complex with an octapeptide

Released:
DOI: 10.2210/pdb1zt1/pdb
PDB entry 1zt1 coloured by chain, front view.
PDB entry 1zt1 coloured by chain, side view.
PDB entry 1zt1 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The H-2Kk MHC peptide-binding groove anchors the backbone of an octameric antigenic peptide in an unprecedented mode.
Kellenberger C, Roussel A, Malissen B
J Immunol 175 3819-25 (2005)
PMID: 16148128

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135072 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, K-K alpha chain Chain: A
Molecule details ›
Chain: A
Length: 277 amino acids
Theoretical weight: 32.33 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P04223 (Residues: 22-297; Coverage: 79%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.84 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Influenza virus epitope, FEANGNLI Chain: P
Molecule details ›
Chain: P
Length: 8 amino acids
Theoretical weight: 877 Da

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P3221
Unit cell:
a: 111.758Å b: 111.758Å c: 109.417Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.212 0.21 0.251
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

What guides MHC-restricted TCR recognition?
Mazza et al. (2007)
4 other articles

5 mentions without citation

Narrow groove and restricted anchors of MHC class I molecule BF2*0401 plus peptide transporter restriction can explain disease susceptibility of B4 chickens.
Zhang et al. (2012)
4 more