› 1zux

X-ray diffraction
1.85Å resolution

EosFP Fluorescent Protein- Green Form

Released:
DOI: 10.2210/pdb1zux/pdb
PDB entry 1zux coloured by chain, front view.
PDB entry 1zux coloured by chain, side view.
PDB entry 1zux coloured by chain, top view.
Source organism: Lobophyllia hemprichii
Primary publication:
Structural basis for photo-induced protein cleavage and green-to-red conversion of fluorescent protein EosFP.
Nienhaus K, Nienhaus GU, Wiedenmann J, Nar H
Proc Natl Acad Sci U S A 102 9156-9 (2005)
PMID: 15964985

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green to red photoconvertible GFP-like protein EosFP Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 224 amino acids
Theoretical weight: 25.81 KDa
Source organism: Lobophyllia hemprichii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5S6Z9 (Residues: 1-226; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CR8 4 x CR8

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 73.27Å b: 106.48Å c: 121.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.242
Expression system: Escherichia coli

Quick links

Citations

35 review citations

Fluorescent proteins and their applications in imaging living cells and tissues.
Chudakov et al. (2010)
34 more

6 mentions without citation

A bright and photostable photoconvertible fluorescent protein.
McKinney et al. (2009)
5 more