Function and Biology Details
Reaction catalysed:
ATP-dependent breakage, passage and rejoining of double-stranded DNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- DNA topoisomerase, type IIA, domain A
- DNA gyrase/topoisomerase IV, subunit A, C-terminal repeat
- DNA gyrase/topoisomerase IV, subunit A, C-terminal
- DNA topoisomerase, type IIA, alpha-helical domain superfamily
- DNA topoisomerase, type IIA, domain A, alpha-beta
- DNA topoisomerase, type IIA-like domain superfamily
- DNA topoisomerase IV, subunit A, Gram-negative
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
DNA topoisomerase 4 subunit A (preferred)
PDBe Complex ID:
PDB-CPX-142727 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA topoisomerase 4 subunit A
Molecule details ›
Chain: A
Length: 716 amino acids
Theoretical weight: 79.77 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
Sequence domains:
Structure domains: Topoisomerase, domain 3
Length: 716 amino acids
Theoretical weight: 79.77 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
- Canonical:
P0AFI2 (Residues: 27-742; Coverage: 95%)
Sequence domains:
Structure domains: Topoisomerase, domain 3
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
ALS BEAMLINE 8.3.1
Spacegroup:
P21212
Expression system: Escherichia coli BL21
