› 1zxx

X-ray diffraction
1.85Å resolution

The crystal structure of phosphofructokinase from Lactobacillus delbrueckii

Released:
DOI: 10.2210/pdb1zxx/pdb
PDB entry 1zxx coloured by chain, front view.
PDB entry 1zxx coloured by chain, side view.
PDB entry 1zxx coloured by chain, top view.
Source organism: Lactobacillus delbrueckii subsp. bulgaricus
Primary publication:
Kinetic and structural characterization of phosphofructokinase from Lactobacillus bulgaricus.
Paricharttanakul NM, Ye S, Menefee AL, Javid-Majd F, Sacchettini JC, Reinhart GD
Biochemistry 44 15280-6 (2005)
PMID: 16285731

Function and Biology Details

Reaction catalysed: 
ATP + beta-D-fructose 6-phosphate = ADP + beta-D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent 6-phosphofructokinase Chain: A
Molecule details ›
Chain: A
Length: 319 amino acids
Theoretical weight: 34.05 KDa
Source organism: Lactobacillus delbrueckii subsp. bulgaricus
Expression system: Escherichia coli
UniProt:
  • Canonical: P80019 (Residues: 1-319; Coverage: 100%)
Gene name: pfkA
Sequence domains: Phosphofructokinase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P6222
Unit cell:
a: 135.014Å b: 135.014Å c: 77.66Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.222 0.251
Expression system: Escherichia coli

Quick links

Citations

9 citation in other articles

Structures of human phosphofructokinase-1 and atomic basis of cancer-associated mutations.
Webb et al. (2015)
8 more

1 mention without citation

Structure of the apo form of Bacillus stearothermophilus phosphofructokinase.
Mosser et al. (2012)