› 1zy2

X-ray diffraction
3.03Å resolution

Crystal structure of the phosphorylated receiver domain of the transcription regulator NtrC1 from Aquifex aeolicus

Released:
DOI: 10.2210/pdb1zy2/pdb
PDB entry 1zy2 coloured by chain, front view.
PDB entry 1zy2 coloured by chain, side view.
PDB entry 1zy2 coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Negative regulation of AAA + ATPase assembly by two component receiver domains: a transcription activation mechanism that is conserved in mesophilic and extremely hyperthermophilic bacteria.
Doucleff M, Chen B, Maris AE, Wemmer DE, Kondrashkina E, Nixon BT
J Mol Biol 353 242-55 (2005)
PMID: 16169010

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130469 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator (NtrC family) Chains: A, B
Molecule details ›
Chains: A, B
Length: 150 amino acids
Theoretical weight: 17.52 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67198 (Residues: 1-136; Coverage: 31%)
Gene names: aq_1117, ntrC1
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand PHD 2 x PHD

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P3121
Unit cell:
a: 91.517Å b: 91.517Å c: 130.942Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.254 0.251 0.283
Expression system: Escherichia coli

Quick links

Citations

9 review citations

The origin of protein interactions and allostery in colocalization.
Kuriyan et al. (2007)
8 more

5 mentions without citation

The role of bacterial enhancer binding proteins as specialized activators of σ54-dependent transcription.
Bush et al. (2012)
4 more