› 1zy9

X-ray diffraction
2.34Å resolution

Crystal structure of Alpha-galactosidase (EC 3.2.1.22) (Melibiase) (tm1192) from Thermotoga maritima at 2.34 A resolution

Released:
DOI: 10.2210/pdb1zy9/pdb
PDB entry 1zy9 coloured by chain, front view.
PDB entry 1zy9 coloured by chain, side view.
PDB entry 1zy9 coloured by chain, top view.
Source organism: Thermotoga maritima
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124427 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-galactosidase Chain: A
Molecule details ›
Chain: A
Length: 564 amino acids
Theoretical weight: 65.67 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: G4FEF4 (Residues: 1-552; Coverage: 100%)
Gene names: TM_1192, Tmari_1199, galA
Sequence domains: Melibiase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand EDO 4 x EDO
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 10 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: C2221
Unit cell:
a: 121.729Å b: 160.533Å c: 88.258Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.162 0.213
Expression system: Escherichia coli

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