› 1zyc

X-ray diffraction
3Å resolution

Crystal Structure of eIF2alpha Protein Kinase GCN2: Wild-Type in Apo Form.

Released:
DOI: 10.2210/pdb1zyc/pdb
PDB entry 1zyc coloured by chain, front view.
PDB entry 1zyc coloured by chain, side view.
PDB entry 1zyc coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural basis for autoinhibition and mutational activation of eukaryotic initiation factor 2alpha protein kinase GCN2.
Padyana AK, Qiu H, Roll-Mecak A, Hinnebusch AG, Burley SK
J Biol Chem 280 29289-99 (2005)
PMID: 15964839

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147365 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
eIF-2-alpha kinase GCN2 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 303 amino acids
Theoretical weight: 35.14 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P15442 (Residues: 594-997; Coverage: 18%)
Gene names: AAS1, GCN2, YDR283C
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 79.89Å b: 95.7Å c: 175.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.299
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Eukaryotic initiation factor 2 phosphorylation and translational control in metabolism.
Baird et al. (2012)
19 more

5 mentions without citation

Substrate and docking interactions in serine/threonine protein kinases.
Goldsmith et al. (2007)
4 more