› 1zyz

X-ray diffraction
2.9Å resolution

Structures of Yeast Ribonucloetide Reductase I

Released:
DOI: 10.2210/pdb1zyz/pdb
PDB entry 1zyz coloured by chain, front view.
PDB entry 1zyz coloured by chain, side view.
PDB entry 1zyz coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structures of eukaryotic ribonucleotide reductase I provide insights into dNTP regulation.
Xu H, Faber C, Uchiki T, Fairman JW, Racca J, Dealwis C
Proc Natl Acad Sci U S A 103 4022-7 (2006)
PMID: 16537479

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-149282 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase large chain 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 888 amino acids
Theoretical weight: 99.67 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P21524 (Residues: 1-888; Coverage: 100%)
Gene names: CRT7, RIR1, RNR1, SDS12, YER070W
Sequence domains:
Structure domains: Anaerobic Ribonucleotide-triphosphate Reductase Large Chain

Ligands and Environments

1 bound ligand:
Ligand GLY 2 x GLY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: F4132
Unit cell:
a: 433.696Å b: 433.696Å c: 433.696Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.217 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

DNA building blocks: keeping control of manufacture.
Hofer et al. (2012)
8 more

4 mentions without citation

When Order Meets Disorder: Modeling and Function of the Protein Interface in Fuzzy Complexes.
Sacquin-Mora et al. (2021)
3 more